Arginine-Modified Hemin Enhances G-Quadruplex DNAzyme Peroxidase Activity for High Sensitivity Detection - Département de biologie
Article Dans Une Revue Analytical Chemistry Année : 2024

Arginine-Modified Hemin Enhances G-Quadruplex DNAzyme Peroxidase Activity for High Sensitivity Detection

Bin Liu
  • Fonction : Auteur
Tian Wang
  • Fonction : Auteur
Dehui Qiu
Xinrong Yan
  • Fonction : Auteur
Yuan Liu
  • Fonction : Auteur
Xiaobo Zhang
Huangxian Ju
Jun Zhou

Résumé

Hemin/G-quadruplex (hG4) complexes are frequently used as artificial peroxidase-like enzymatic systems (referred to as G4 DNAzymes) in many biosensing applications, in spite of a rather low efficiency, notably in terms of detection limits. To tackle this issue, we report herein on a strategy in which hemin is chemically modified with the amino acids found in the active site of parent horseradish peroxidase (HRP), in the aim of recreating an environment conducive to a high catalytic activity. When hemin conjugated with a single arginine, it associates with G4 to create an arginine-hemin/G4 (R-hG4) DNAzyme that exhibits improved catalytic performances, characterized by kinetic analysis and DFT calculations. The practical relevance of this system was demonstrated with the implementation of biosensing assays enabling the chemiluminescent detection of G4-containing DNA and colorimetry detection of FEN1 enzyme with a high efficiency and sensitivity. Our results thus provide a guide for future enzyme engineering campaigns to create ever more efficient peroxidase-mimicking DNA-based systems.
Fichier sous embargo
Fichier sous embargo
0 5 5
Année Mois Jours
Avant la publication
mardi 25 février 2025
Fichier sous embargo
mardi 25 février 2025
Connectez-vous pour demander l'accès au fichier

Dates et versions

hal-04678260 , version 1 (05-09-2024)

Identifiants

Citer

Bin Liu, Tian Wang, Dehui Qiu, Xinrong Yan, Yuan Liu, et al.. Arginine-Modified Hemin Enhances G-Quadruplex DNAzyme Peroxidase Activity for High Sensitivity Detection. Analytical Chemistry, In press, Online ahead of print. ⟨10.1021/acs.analchem.4c03013⟩. ⟨hal-04678260⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

More